Dictionary of Human Evolution and Biology

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DNA Sequencing Techniques

Analytical procedure that consists of (a) selection of DNA clones; (b) causing base-specific reactions to occur; (c) separation by fragment size on sequencing gels; and (d) automated reading of the banded gels as a sequence of A, T, G, and C bases. There are two common methods: the chain termination method of Sanger and colleagues, and the chemical method of Maxam and Gilbert. Hood and colleagues developed automation devices in the mid-1980s, and this final step made the Human Genome Project feasible. Aka DNA sequence analysis, nucleotide sequencing.

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